In this study, a facile method to fabricate Janus microbeads based on photonic crystals and upconversion nanoparticles is designed. The Janus microbeads can be reversed under magnetic response and generate upconversion fluorescence under near-infrared light. Three kinds of core-shell upconversion nanoparticles (UCNPs) are prepared by the solvothermal method and are mixed with Fe3O4 nanoparticles and different sizes of colloidal spheres. The Janus microbeads are assembled according to the hydrophilic property of the mixture and the hydrophobic property of substrates. The upper parts of the Janus microbeads are photonic crystals assembled with colloidal spheres, and the other parts are Fe3O4. Meanwhile, UCNPs are distributed inside the Janus microbeads. Furthermore, the Janus microbeads are prepared into different lattice patterns using special templates. In the lattice patterns, the structural colors of Janus microbeads can be displayed and disappeared by magnetic field inversion, and under external NIR irradiation, Janus microbeads can generate upconversion fluorescence to achieve multiple color display. The Janus microbeads are also applied to both sides of the bank card, and various reading information methods are designed according to different response modes, which have important applications in pattern display, response materials, and anti-counterfeiting. (C) 2021 Elsevier Inc. All rights reserved.