Inspired by the biomimetic modification strategy of dopamine self-polymerization technique, molecularly imprinted nanocomposite membranes (MINCMs) with thermo-responsive rebinding and separation perfor-mance were synthesized and evaluated. Herein, the Au/SiO2-based multilevel structure had been successfully obtained onto the polydopamine (pDA) modified membrane surfaces. Afterward, the poly(Nisopropylacrylamide)-based biomolecule-imprinted sites were adequately constructed by developing a photoinitiated atom transfer radical polymerization (pATRP) imprinting strategy using the high-biocompatible ovalbumin (Ova, pI 4.6) as template molecule. Therefore, thermo-responsive 'specific recognition sites' toward Ova were then achieved on the as-prepared MINCMs after the well-designed imprinting process. When the external temperature was set at 37 degrees C, excellent ovalbumin rebinding capacity (33.26 mg/g), selectivity factor (3.06) and structural stability were obtained. Importantly, as to the controllable biocompatibility research of this work, the bare glass and Ova-bound-MINCMs (the MINCMs were bound with Ova) showed basically the same cell adhesion behaviors and viability, indicating the excellent biocompatibility of the Ovabound-MINCMs. Additionally, efficient and rapid regulation of cell adhesion/detachment on ovalbuminbound MINCMs could be still obtained even after 10 cycles of temperature-switch process, which indicated that the as-prepared MINCMs had strong ability to work under high intensity and long continuous operation. (c) 2020 Elsevier Inc. All rights reserved.