Searching for novel sensing material for highly sensitive nonenzymatic detection of glucose is important. In this paper, it is the first time that AgCoO2/MWCNTs was exploring as electrocatalyst for glucose oxidation. Structural characterizations reveal that AgCoO2 and MWCNTs are interconnected with a three-dimensional porous structure, which leads to attractive electrocatalytic activity for glucose oxidation. The sensor based on AgCoO2/MWCNTs is found to exhibit excellent analytical performances for glucose with an extremely high sensitivity of 8214.3 mu A mM(-1) cm(-2) and a wide linear range of four orders of magnitude. Such results indicate that AgCoO2/MWCNTs can be employed as a promising material for electrochemical sensing of glucose and also opens avenues for applications of AgCoO2 in the fields of electrochemical sensing.