An electrochemical biosensor based on a waterstable one-dimensional double-chain Cu(II) metal-organic framework (Cu-MOF) directly was constructed for efficiently recognizing L-tyrosine (L-Tyr) in biomimic environments. Cu-MOF:{[Cu(bpe)(fdc) (H2O)(DMF)]center dot 0.5H(2)O}(n) (bpe = 1,2-di(4-pyridyl)ethylene, H(2)fdc = 2,5-furandicarboxylic acid, namely, Cu-1) was synthesized by a hydrothermal method. It was characterized by IR, scanning electron microscopy, atomic force microscopy, and PXRD techniques. Cu-1 exhibited extreme solvent and thermal stability as well as excellent electroconductive character. It was coated on a glassy carbon electrode (GCE) surface to prepare an electrochemical biosensor (Cu-1/GCE) which showed preferable biosensing ability toward L-Tyr. This Cu-MOF electrochemical biosensor showed simple operation and high sensitivity toward L-Tyr in the concentration range from 0.01 to 0.09 mM. The detection limit is 5.822 mu M. Furthermore, Cu-1/GCE showed extremely excellent selectivity to L-Tyr in a biomimic environment with several amino acid interferents. This new strategy exhibits great potential applications for designing MOFs with excellent electrochemical activity.