Kafirin-based films can be a potential alternative to petroleum-derived plastic and is an essential step towards environmental waste management. This study presents kafirin as a bio-polymer with the potential for making films by using a solution casting technique. Kafirin films with varying concentrations of polyethylene glycol (400), triacetin and triethyl citrate were cast. The interaction of kafirin by the plasticizer was confirmed by FTIR spectra studies. The effect of plasticizers on the crystallinity of the polymers has been studied by using the XRD technique. The change in glass transition temperature (T-g) of the films was analysed using differential scanning calorimetry. The thermal stability of films was ascertained by TGA. The measurement of breaking strength and elongation at break helped in studying the effect of plasticizers on the mechanical properties of the films. The water permeability and water contact angle test have been carried out to understand the barrier properties that are necessary for food-grade applications. By comparing test results, it was found that various plasticizers are suitable to be used with kafirin depending on the end-use application.