Electron energy-loss spectroscopy (EELS) has been applied to in situ prepared (DMe-DCNQI)(2)Cu (DMe-DCNQI=2,5-dimethyl-N,N’-dicyanoquinonediimine). The intensity of the loss peaks changed significantly with the primary electron energies. It is probably due to the matrix element effects of the EELS. In conjunction with photoelectron spectroscopy using synchrotron radiation, the nature of the unoccupied levels has been elucidated.