The impacts of different operational temperatures, and organic load (OL) on the fate of SARS-CoV-2 during the anaerobic co-digestion of food waste (FW) and sewage sludge (SS) was evaluated. The lab-scaled batch reactors (i.e. R1-R7) were performed under psychrophilic, mesophilic, and thermophilic conditions and the OL of systems was 1.5,3.5, 6 gVS/L. The performance parameters showed that at higher OL the stability of systems failed and low biogas was produced. In contrast, increasing of operational temperature of systems induced more biogas generation due to the increment of metabolic activity of bacteria. Therefore, R1-R7 achieved biogas yield of 202.5, 249, 187, 260, 246, 163, and 300 mL/gVS respectively. Both SARS-CoV-2 genes i.e. ORFlab, and N genes were detected in the effluent of psychrophilic reactors i.e. R1, and R2, with a total concentration of 46 x 10(3), and 11 x 10(3) copies/L respectively. In R3, no viral genes were observed, when the VFAs was accumulated up to 2000 mg/L and caused a pH drop to 5.6. At the mesophilic condition, the viral concentration was significantly declined, and no viral genes were observed at an OL of 3.5 gVS/L. Furthermore, the synergistic effect of temperature and accumulation of intermediate metabolites provided a sever condition for SARS-CoV-2 survival at an operational temperature and OL of 50 degrees C, and 1.5 gVS/L respectively. (C) 2020 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.