The biomass of sorghum (Sucrosorgo 506) and hemp (Tygra) were ground on a knife mill, then chem. pretreated with NaOH, tested for cellulolytic and xylanolytic activity and hydrolyzed with enzyme prepns. Flashzyme Plus 200 and Celluclast 1.5 L The amt. of released reducing sugars was detd. by the Miller method. The enzyme complex was then selected for the separate hydrolysis and fermentation process. The enzymatic hydrolysis parameters were detd. by the response plane method, on the basis of the amt. of released glucose. The use of an enzyme complex composed of Flashzyme Plus 200, xylanase and glucosidase resulted in effective conversion of both sorghum and hemp biomass. The glucose contents were 60 g/L (sorghum) and 37 g/L (hemp).