Ceiba pentandra, non-edible oil (acid value of 21 mg KOH/g estimated using ASTM D664 methodology) is employed as a source for producing biodiesel using lipase immobilized on mesoporous material as a catalyst. Optimum conditions for maximum yield (96.4%) were the temperature of 33 degrees C, methanol to oil molar ratio of about 13.3:1 with a water content of 14.5%. From the reusability studies, it can be observed that greater than 85% conversion could be obtained up to 10 cycles, thereby proving the significant efficacy of the catalyst. Density, flash point, cloud point, calorific value, and cetane number of the produced biodiesels were 885 kg/m3, 152 degrees C, -3 degrees C, 38.44 kJ/kg and 57.2, respectively meeting the ASTM standards specified for biodiesel. The performance and emissions characteristics of 20% biodiesel (CIB20) and petroleum diesel were studied in a VCR under varying speeds in a full load condition. Blended biofuel showed a 13% lower mean brake power (BP) and 25% higher mean specific fuel consumption (SFC) compared to diesel fuel. Though NOx emission of the blended diesel was 31% higher than that of petroleum diesel, Hydrocarbon, CO2, and CO emissions were 8.4%, 13.7%, and 5.08% lower than that of diesel fuel, respectively. (C) 2020 Elsevier Ltd. All rights reserved.