Durian peel (DP) is considered as potential renewable resource of lignocelluloses. The utilization of DP for the production of microbial lipid as biodiesel feedstock has not yet been reported. Hence, this study aimed to convert DP hydrolysate (DPH) into microbial lipid by newly isolated yeast R. mucilaginosa KKUSY14. Lipid yield of 1.68 0.24 g/L and 1.68 +/- 0.24 g/L was achieved in undetoxified and detoxified DPH, respectively. The main compositions of fatty acids were consisted of C16-C18 series with high content of oleic acid (51.2-65.7%) with the absence of linolenic acid. Over 90% of lipid in wet cell could be converted into biodiesel and most of biodiesel fuel properties reached the specified limit of ASTM D6751 and EN14214. R. mucilaginosa KKUSY14 has potential to convert 1000 g durian peel into 103.3 g biomass, 16.8 g lipid, and 15.86 g biodiesel. R. mucilaginosa KKUSY14 demonstrated good characteristics as promising yeast capable to convert DPH into lipids and biodiesel without detoxification. (C) 2020 Elsevier Ltd. All rights reserved.