화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.101, 195-204, September, 2021
DOI10.1016/j.jiec.2021.06.012  Export Citation
A microlagal-based carbonaceous sensor for enzymatic determination of glucose in blood serum
Seyed Mostafa Jafari, Saeed Masoum†, and Seyed Ali Hosseini Tafreshi1
  • Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran
  • 1Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran
E-mail:
In the present work, Chlorella vulgaris, a well-known microalga was used as a carbonic precursor, to synthesis the water-soluble carbon dots (CDs) via hydrothermal method. To improve the properties, acidic carbon dots (ACDs) were also synthesized via ultrasoncation of microalgal biomass after acidic hydrolysis (using diluted acidic solution). The aqueous solution of ACDs was used as a sensor for glucose determination based on glucose oxidase enzyme (GOx) reaction and fluorescence quenching of ACDs in the presence of Fe3+ ions under the optimized conditions. The linear range of glucose in the blood serum was measured from a range of 5 mM to 500 mM with a limit of detection (LOD) 2.84 mM. Additionally, the hydrogen peroxide, as the conventional reactive oxygen species (ROS) was determined based on the Fenton reaction. The linear range of H2O2 was obtained from 10 μM to 200 μM with LOD of 975 nM. In all of these applications, fluorescence quenching of ACDs was followed according to photo-induced electron transfer (PET) mechanism in the presence of generated Fe3+ ions by Fenton reaction.
Keywords:Carbonaceous sensor;Microalga;Carbon dots;Fluorescence quenching;Glucose;Reactive oxygen species
  1. Baker SN, Baker GA, Angew. Chem.-Int. Edit., 49, 6726 (2010)
  2. Yang Z, Li Z, Xu M, Ma Y, Zhang J, Su Y, Gao F, Wei H, Zhang L, Nano-Micro Lett., 5, 247 (2013)
  3. Lu W, Qin X, Liu S, Chang G, Zhang Y, Luo Y, Asiri AM, Al-Youbi AO, Sun X, Anal. Chem., 84, 5351 (2012)
  4. Huang H, Li CG, Zhu SJ, Wang HL, Chen CL, Wang ZR, Bai TY, Shi Z, Feng SH, Langmuir, 30(45), 13542 (2014)
  5. Kang YF, Li YH, Fang YW, Xu Y, Wei XM, Yin XB, Sci. Rep., 5, 11835 (2015)
  6. Yang ZC, Wang M, Yong AM, Wong SY, Zhang XH, Tan H, Chang AY, Li X, Wang J, Chem. Commun., 47, 11615 (2011)
  7. Kumar K, Toffoli G, Rizzolio F, Fluorescent Carbon Nanoparticles in Medicine for Cancer Therapy. ACS Publications, 2013.
  8. Ding C, Zhu A, Tian Y, Accounts Chem. Res., 47, 20 (2013)
  9. Anwar S, Ding H, Xu M, Hu X, Li Z, Wang J, Liu L, Jiang L, Wang D, Dong C, ACS Appl. Bio Mater., 2, 2317 (2019)
  10. Li M, Chen T, Gooding JJ, Liu J, ACS Sensors, 4, 1732 (2019)
  11. Ding C, Zhu A, Tian Y, Accounts Chem. Res., 47, 20 (2014)
  12. Wang JM, Xu MS, Wang D, Li ZZ, Primo FL, Tedesco AC, Bi H, Inorg. Chem., 58(19), 13394 (2019)
  13. Bhattacharya S, Sarkar R, Nandi S, Porgador A, Jelinek R, Anal. Chem., 89, 830 (2016)
  14. Trachootham D, Alexandre J, Huang P, Nat. Rev. Drug Discovery, 8, 579 (2009)
  15. Barnham KJ, Masters CL, Bush AI, Nat. Rev. Drug Discovery, 3, 205 (2004)
  16. Fraisl P, Aragones J, Carmeliet P, Nat. Rev. Drug Discovery, 8, 139 (2009)
  17. Niedowicz DM, Daleke DL, Cell Biochem. Biophys., 43, 289 (2005)
  18. Butterfield DA, Chem. Res. Toxicol., 10, 495 (1997)
  19. Jenner P, Ann. Neurol., 53, S26 (2003)
  20. Ju E, Liu Z, Du Y, Tao Y, Ren J, Qu X, ACS Nano, 8, 6014 (2014)
  21. Qu S, Chen H, Zheng X, Cao J, Liu X, Nanoscale, 5, 5514 (2013)
  22. Tong G, Wang J, Wang R, Guo X, He L, Qiu F, Wang G, Zhu B, Zhu X, Liu T, J. Mater. Chem. B, 3, 700 (2015)
  23. Pakkath SAR, Chetty SS, Selvarasu P, Murugan AV, Kumar Y, Periyasamy L, Santhakumar M, Sadras SR, Santhakumar K, ACS Biomater. Sci. Eng., 4, 2582 (2018)
  24. Wei J, Qiang L, Ren J, Ren X, Tang F, Meng X, Anal. Methods, 6, 1922 (2014)
  25. Wang L, Zheng J, Li Y, Yang S, Liu C, Xiao Y, Li J, Cao Z, Yang R, Anal. Chem., 86, 12348 (2014)
  26. Shen P, Xia Y, Anal. Chem., 86, 5323 (2014)
  27. Wu Y, Wei P, Pengpumkiat S, Schumacher EA, Remcho VT, Anal. Chem., 87, 8510 (2015)
  28. Loo AH, Sofer Z, Bousa D, Ulbrich P, Bonanni A, Pumera M, ACS Appl. Mater. Interfaces, 8, 1951 (2016)
  29. Yap SHK, Chan KK, Zhang G, Tjin SC, Yong KT, ACS Appl. Mater. Interfaces, 11, 28546 (2019)
  30. Chatzimitakos TG, Kasouni AI, Troganis AN, Stalikas CD, ACS Appl. Mater. Interfaces, 10, 16024 (2018)
  31. Zheng M, Xie Z, Qu D, Li D, Du P, Jiang X, Sun Z, ACS Appl. Mater. Interfaces, 5, 13242 (2013)
  32. Jiang Y, Wang Z, Dai Z, ACS Appl. Mater. Interfaces, 8, 3644 (2015)
  33. Dong Y, Wang R, Li G, Chen C, Chi Y, Chen G, Anal. Chem., 84, 6220 (2012)
  34. Zhang Z, Shi Y, Pan Y, Cheng X, Zhang L, Chen J, Li MJ, Yi C, J. Mater. Chem. B, 2, 5020 (2014)
  35. Zhang J, Chen X, Li Y, Han S, Du Y, Liu H, Anal. Methods, 10, 541 (2018)
  36. Li H, Zhai J, Tian J, Luo Y, Sun X, Biosens. Bioelectron., 26, 4656 (2011)
  37. Ma JL, Yin BC, Wu X, Ye BC, Anal. Chem., 89, 1323 (2016)
  38. Lan D, Li B, Zhang Z, Biosens. Bioelectron., 24, 934 (2008)
  39. Shafer-Peltier KE, Haynes CL, Glucksberg MR, Van Duyne RP, J. Am. Chem. Soc., 125(2), 588 (2003)
  40. Chen R, Xu W, Xiong C, Zhou X, Xiong S, Nie Z, Mao L, Chen Y, Chang HC, Anal. Chem., 84, 465 (2011)
  41. Xia Y, Ye J, Tan K, Wang J, Yang G, Anal. Chem., 85, 6241 (2013)
  42. Cao X, Wang N, Jia S, Shao Y, Anal. Chem., 85, 5040 (2013)
  43. Li J, Li Y, Shahzad SA, Chen J, Chen J, Wang Y, Yang M, Yu C, Chem. Commun., 51, 6354 (2015)
  44. Andrade L, Andrade C, Dias M, Nascimento C, Mendes M, Nutraceut. Food Suppl., 45 (2018).
  45. Park C, Lee JH, Yang X, Yoo HY, Lee JH, Lee SK, Kim SW, Bioprocess. Biosyst. Eng., 39, 1015 (2016)
  46. Guo LP, Zhang Y, Li WC, J. Colloid Interface Sci., 493, 257 (2017)
  47. Zhou J, Deng W, Wang Y, Cao X, Chen J, Wang Q, Xu W, Du P, Yu Q, Chen J, Acta Biomater., 42, 209 (2016)
  48. Zu F, Yan F, Bai Z, Xu J, Wang Y, Huang Y, Zhou X, Microchim. Acta, 184, 1899 (2017)
  49. Sambusiti C, Bellucci M, Zabaniotou A, Beneduce L, Monlau F, Renew. Sust. Energ. Rev., 44, 20 (2015)
  50. Steiner MS, Duerkop A, Wolfbeis OS, Chem. Soc. Rev., 40, 4805 (2011)
  51. Sajid P, Chetty SS, Praneetha S, Murugan AV, Kumar Y, Periyasamy L, RSC Adv., 6 (2016)
  52. Ding H, Yu SB, Wei JS, Xiong HM, ACS Nano, 10, 484 (2015)
  53. Singh RK, Patel KD, Mahapatra C, Kang MS, Kim HW, ACS Appl. Mater. Interfaces, 8, 24433 (2016)
  54. He D, Zheng C, Wang Q, He C, Lee YI, Wu L, Hou X, Talanta, 142, 51 (2015)
  55. Song Y, Zhu S, Xiang S, Zhao X, Zhang J, Zhang H, Fu Y, Yang B, Nanoscale, 6, 4676 (2014)
  56. Ding H, Yu SB, Wei JS, Xiong HM, ACS Nano, 10, 484 (2016)
  57. Bhattacharya S, Sarkar R, Nandi S, Porgador A, Jelinek R, Anal. Chem., 89, 830 (2017)
  58. Qu F, Guo X, Liu D, Chen G, You J, Sens. Actuators B-Chem., 233, 320 (2016)
  59. Jiang H,, Chen Z, Cao H, Huang Y, Analyst, 137, 5560 (2012)
  60. Xu Y, Pehrsson PE, Chen L, Zhang R, Zhao W, J. Phys. Chem. C, 111, 8638 (2007)
  61. Luo Z, Yuwen L, Han Y, Tian J, Zhu X, Weng L, Wang L, Biosens. Bioelectron., 36, 179 (2012)
  62. Zheng AX, Cong ZX, Wang JR, Li J, Yang HH, Chen GN, Biosens. Bioelectron., 49, 519 (2013)
  63. Xu C, Ren J, Feng L, Qu X, Chem. Commun., 48, 3739 (2012)
  64. Wang LL, Qiao J, Liu HH, Hao J, Qi L, Zhou XP, Li D, Nie ZX, Mao LQ, Anal. Chem., 86, 9758 (2014)
  65. Zhang L, Zhang ZY, Liang RP, Li YH, Qiu JD, Anal. Chem., 86, 4423 (2014)
  66. Yi Y, Deng J, Zhang Y, Li H, Yao S, Chem. Commun., 49, 612 (2013)
  67. Ma JL, Yin BC, Wu X, Ye BC, Anal. Chem., 89, 1323 (2017)
  68. Kong KV, Lam ZY, Lau WKO, Leong WK, Oivo M, J. Am. Chem. Soc., 135(48), 18028 (2013)