Optimization study for enhanced production of hyaluronic acid from Streptococcus equisimilus MK156140

Ashwini Kumar; Savitha Janakiraman; Lokesh Kyathsandra Nataraj

Korean Journal of Chemical Engineering, Vol.38, No.9, 1880-1887, September, 2021

DOI10.1007/s11814-021-0798-0 Export Citation

Optimization study for enhanced production of hyaluronic acid from Streptococcus equisimilus MK156140

Ashwini Kumar, Savitha Janakiraman^†, and Lokesh Kyathsandra Nataraj¹

Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bangalore-560 056, India
¹M.S. Ramaiah Institute of Technology, Bangalore-560054, India

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Hyaluronic acid finds its complete application in areas such as therapeutics, cosmetics, and as a health supplement. In the present investigation, standardization for the production of hyaluronic acid by Streptococcus equisimilus MK156140 in complex media was performed. Some of the selected physicochemical parameters such as pH, temperature, speed, incubation time, sucrose, yeast extract, and beef extract were screened using Plackett-Burman foldover design. Further, the screened parameters interaction was investigated using central composite design (CCD) and closely compared with OVAT studies. At a pH of 7.38, with beef extract, 12.15%, and yeast extract 7.64%, the observed yield was 7.16 g/L, which was in close line with the predicted value of 7.21 g/L.

Keywords:Response Surface Methodology;OVAT;Plackett Burman Design;Central Composite Design

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[6] Chong BF, Blank LM, Mclaughlin R, Nielsen LK, Appl. Microbiol. Biotechnol., 66(4), 341 (2005)

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[9] Blank LM, McLaughlin RL, Nielsen LK, Biotechnol. Bioeng., 90(6), 685 (2005)

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