바이오매스 유래 파클리탁셀의 경제적인 회수를 위한 물과 헥산의 순차적 세척 방법

이명기; 김진현; Myeong-Gi Lee; Jin-Hyun Kim

Korean Chemical Engineering Research, Vol.60, No.1, 169-174, February, 2022

DOI10.9713/kcer.2022.60.1.169 Export Citation

바이오매스 유래 파클리탁셀의 경제적인 회수를 위한 물과 헥산의 순차적 세척 방법

A Tandem Water and Hexane Washing Method for Economical Recovery of Paclitaxel from Biomass

이명기, 김진현 ^†

공주대학교 화학공학부, 31080 충남 천안시 서북구 천안대로 1223-24

Myeong-Gi Lee, and Jin-Hyun Kim^†

Department of Chemical Engineering, Kongju National University, Cheonan, 31080, Korea

E-mail:

초록

본 연구에서는 Taxus chinensis 유래 파클리탁셀의 회수 효율을 개선하기 위하여, 물 및 헥산의 순차적 세척 공정을 개발하였다. 물 세척은 시료/물 비 1:40 (w/v)에서 10분 동안 세척함으로써 시료에 포함되어 있는 극성불순물을 효과적으로 제거할 수 있었다. 헥산 세척은 시료/헥산 비 1:160 (w/v)에서 20 분 동안 세척함으로써 시료에 포함되어 있는 비극성 불순물을 효과적으로 제거할 수 있었다. 물과 헥산의 순차적 세척을 통하여 비교적 짧은 공정 시간(~30분)에 파클리탁셀 순도 30% 이상을 얻을 수 있었다.

In this study, a tandem water and hexane washing process was developed to improve the recovery efficiency of paclitaxel derived from Taxus chinensis. The polar impurities contained in the sample were effectively removed by washing with water at a sample/water ratio of 1:40 (w/v) for 10 min. In addition, the non-polar impurities were effectively removed by washing with hexane at a sample/hexane ratio of 1:160 (w/v) for 20 min. A high purity of paclitaxel (>30.0%) was obtained in a short operating time (~30 min) by sequential washing with water and hexane.

Keywords:Paclitaxel;Purification;Tandem washing;Water;Hexane;Process optimization

[References]

Park JM, Kim JH, Korean Chem. Eng. Res., 59(1), 106 (2021)
Zhu L, Chen L, Cell. Mol. Biol. Lett., 24, 40 (2019)
Tan Z, Li Q, Wang C, Zhou W, Yang Y, Wang H, Yi Y, Li F, Molecules, 22, 1483 (2017)
Wei Y, Pu X, Zhao L, Oncol. Rep., 37, 3159 (2017)
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Oguzkan SB, Karagul B, Uzun A, Guler OO, Ugras HI, Int. J. Pharmacol., 14, 76 (2018)
Ochoa-Villarreal M, Howat S, Hong S, Jang MO, Jin YW, Lee EK, Loake GJ, BMB Rep., 49, 149 (2016)
Pyo SH, Park HB, Song BK, Han BH, Kim JH, Process Biochem., 39(12), 1985 (2004)
McPartland TJ, Patil RA, Malone MF, Roberts SC, Biotechnol. Prog., 28(4), 990 (2012)
Kang HJ, Kim JH, Biotechnol. Bioprocess Eng., 24, 513 (2019)
Oh SR, Kim JH, Korean J. Chem. Eng., 38(3), 480 (2021)
Lee CG, Kim JH, Process Biochem., 59, 216 (2017)
Lee CG, Kim JH, Process Biochem., 51(10), 1738 (2016)
Panchal B, Deshmukh S, Sharma M, Int. J. Oil, Gas Coal Eng., 2, 1 (2016)

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[1] Park JM, Kim JH, Korean Chem. Eng. Res., 59(1), 106 (2021)

[2] Zhu L, Chen L, Cell. Mol. Biol. Lett., 24, 40 (2019)

[3] Tan Z, Li Q, Wang C, Zhou W, Yang Y, Wang H, Yi Y, Li F, Molecules, 22, 1483 (2017)

[4] Wei Y, Pu X, Zhao L, Oncol. Rep., 37, 3159 (2017)

[5] Zu YG, Wang Y, Fu YJ, Li SM, Sun R, Liu W, Luo H, Sep. Purif. Technol., 68(2), 238 (2009)

[6] Oguzkan SB, Karagul B, Uzun A, Guler OO, Ugras HI, Int. J. Pharmacol., 14, 76 (2018)

[7] Ochoa-Villarreal M, Howat S, Hong S, Jang MO, Jin YW, Lee EK, Loake GJ, BMB Rep., 49, 149 (2016)

[8] Pyo SH, Park HB, Song BK, Han BH, Kim JH, Process Biochem., 39(12), 1985 (2004)

[9] McPartland TJ, Patil RA, Malone MF, Roberts SC, Biotechnol. Prog., 28(4), 990 (2012)

[10] Kang HJ, Kim JH, Biotechnol. Bioprocess Eng., 24, 513 (2019)

[11] Oh SR, Kim JH, Korean J. Chem. Eng., 38(3), 480 (2021)

[12] Lee CG, Kim JH, Process Biochem., 59, 216 (2017)

[13] Lee CG, Kim JH, Process Biochem., 51(10), 1738 (2016)

[14] Panchal B, Deshmukh S, Sharma M, Int. J. Oil, Gas Coal Eng., 2, 1 (2016)