Performance Improvement of Hydrophobized Bacterial Cellulose Films as Wound Dressing

Katlyn Bazoli dos Santos; Gustavo Eiji Higawa; Karen Stefany Conceição; Denise Coutinho Endringer; Elisangela Flavia Pimentel Schmitt; Lorena Martins Xavier; Marcio Fronza; Alessandra Stevanato; Cesar Augusto Tischer; Renato Márcio Ribeiro-Viana

Macromolecular Research, Vol.30, No.2, 116-123, February, 2022

DOI10.1007/s13233-022-0005-0 Export Citation

Performance Improvement of Hydrophobized Bacterial Cellulose Films as Wound Dressing

Katlyn Bazoli dos Santos¹, Gustavo Eiji Higawa², Karen Stefany Conceição³, Denise Coutinho Endringer⁴, Elisangela Flavia Pimentel Schmitt⁴, Lorena Martins Xavier⁴, Marcio Fronza⁴, Alessandra Stevanato², Cesar Augusto Tischer³, and Renato Márcio Ribeiro-Viana^{1, 5, †}

¹Programa de Pós-graduação em Ciência e Engenharia de Materiais (PPGCEM), Universidade Tecnológica Federal do Paraná, UTFPR-Ld, CEP, 86036-370, Londrina, PR, Brazil
²Departamento Acadêmico de Química, Universidade Tecnológica Federal do Paraná, UTFPR-Ld, CEP, 86036-370, Londrina, PR, Brazil
³Departamento de Bioquímica e Biotecnologia, Universidade Estadual de Londrina, UEL, CEP 86051-980, Londrina, PR, Brazil
⁴Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Vila Velha — UVV, CEP 29102-920, Vila Velha, ES, Brazil
⁵Departamento Acadêmico de Química,, Universidade Tecnológica Federal do Paraná, 86036-370, Londrina, PR, Brazil

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In the wound dressing research field, there is a continuous search for high-quality materials which present properties superior to those already used. Bacterial cellulose films are recognized as being effective, but their performance can still be further enhanced. On the other hand, wound dressings which present surfaces modified with hydrophobic molecules, such as dialkyl carbamoyl chloride, appear to be an alternative material, acting as antimicrobial dressings. Based on that, this paper describes the synthesis of small hydrophobic molecules based on inexpensive alcohols, such as octyl and benzyl alcohol, conjugated to the hydroxyl groups of bacterial cellulose. The films were prepared using ultrasound irradiation and characterized via infrared, as well as for their wettability and water absorption capacity, which showed greater contact angles and similar moisture retention when compared to unmodified films. Morphological aspects of modified films were analyzed by scanning electron microscope (SEM) and minimal modification in the structure was found. The hydrophobized cellulose films showed cytocompatibility with fibroblasts, and antimicrobial activity when compared to native bacterial cellulose films, by reducing the bacterial load up to 75%. This type of modification on these films is of interest in order to prepare films with better properties as dressings based on bacterial cellulose.

Keywords:wound dressing;chemical modification;bacterial cellulose;hydrophobic;biomaterial

[References]

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[1] Sulaeva I, Henniges U, Rosenau T, Potthast A, Biotechnol. Adv., 33, 1547 (2015)

[2] Homaeigohar S, Boccaccini AR, Acta Biomater., 107, 25 (2020)

[3] Aavani F, Khorshidi S, Karkhaneh A, J. Med. Eng. Technol., 43, 38 (2019)

[4] Kalantari K, Mostafavi E, Afifi AM, Izadiyan Z, Jahangirian H, Rafiee-Moghaddam R, Webster TJ, Nanoscale, 12, 2268 (2020)

[5] Chadwick P, Ousey K, J. Wound Care, 28, 370 (2019)

[6] Wadstr?m T, Bj?rnberg S, Hjert?n S, Acta Pathol. Microbiol. Scand. Ser. B Microbiol., 93B, 359 (1985)

[7] Braunwarth H, Brill FHH, Wound Med., 5, 16 (2014)

[8] Zheng Z, Xu Q, Guo J, Qin J, Mao H, Wang B, Yan F, ACS Appl. Mater. Interfaces, 8, 12684 (2016)

[9] Wiener MC, Horanyi PS, Om T, Om T, Proc. Natl. Acad. Sci., 108, 10929 (2011)

[10] Fu L, Zhang J, Yang G, Carbohydr. Polym., 92, 1432 (2013)

[11] Shah N, Ul-Islam M, Khattak WA, Park JK, Carbohydr. Polym., 98, 1585 (2013)

[12] Shoda M, Sugano Y, Petersen N, Gatenholm P, Appl. Microbiol. Biotechnol., 91, 1277 (2011)

[13] Abushammala H, Mao J, Molecules, 24, 2782 (2019)

[14] Habibi Y, Chem. Soc. Rev. , 43, 1519 (2014)

[15] Parte FGB, Santoso SP, Chou CC, Verma V, Wang HT, Ismadji S, Cheng KC, Crit. Rev. Biotechnol., 40, 397 (2020)

[16] Wu J, Zheng Y, Song W, Luan J, Wen X, Wu Z, Chen X, Wang Q, Guo S, Carbohydr. Polym., 102, 762 (2014)

[17] de Mattos IB, Nischwitz SP, Tuca AC, Groeber-Becker F, Funk M, Birngruber T, Mautner SI, Kamolz LP, Holzer JCJ , Burns, 46, 918 (2020)

[18] Ahamed MIN, Sankar S, Kashif PM, Basha SKH, Sastry TP, Int. J. Biol. Macromol., 72, 680 (2015)

[19] Cacicedo ML, Pacheco G, Islan GA, Alvarez VA, Barud HS, Castro GR, Int. J. Biol. Macromol., 147, 1136 (2020)

[20] Namazi H, Rakhshaei R, Hamishehkar H, Kafil HS, Int. J. Biol. Macromol., 87, 327 (2016)

[21] Orlando U, Basnett P, Nigmatullin R, Wang W, Knowles JC, Roy I, Front. Bioeng. Biotechnol., 8, 1 (2020)

[22] Goelzer FDE, Faria-Tischer PCS, Vitorino JC, Sierakowski MR, Tischer CA, Mater. Sci. Eng. C, 29, 549 (2009)

[23] Mosmann T, J. Immunol. Methods, 65, 55 (1983)

[24] CLSI, Performance Standards for Antimicrobial Susceptibility Testing. Informational Supplement, Clinical & Laboratory Standards Institute.

[25] Birkheur S, Laureto E, Fernandes RV, Tischer C, Butera AP, Ribeiro-Viana RM, Mater. Res., 23, 20200197 (2020)

[26] Kaewnopparat S, Sansernluk K, Faroongsarng D, AAPS PharmSciTech, 9, 701 (2008)

[27] Chenab KK, Sohrabi B, Rahmanzadeh A, Biomater. Sci., 7, 3110 (2019)

[28] Song R, Zhang Y, Huang Q, Yang Y, Lin L, Liang J, Hu R, Rui G, Lin C, ACS Appl. Bio Mater., 1, 1056 (2018)