Oxidized Alginate Hydrogel-Based Derivatives with Optimized Features for Cell Culture Scaffold

Etienne Twizeyimana; Shuang Zhang; Jean Felix Mukerabigwi; Zhishen Ge

Macromolecular Research, Vol.30, No.4, 238-244, April, 2022

DOI10.1007/s13233-022-0030-z Export Citation

Oxidized Alginate Hydrogel-Based Derivatives with Optimized Features for Cell Culture Scaffold

Etienne Twizeyimana¹, Shuang Zhang¹, Jean Felix Mukerabigwi^{1, 2, †}, and Zhishen Ge¹

¹CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, Anhui, P. R. China
²Department of Chemistry, College of Science and Technology, University of Rwanda, Po. Box: 3900 Kigali, Rwanda

E-mail:

Advances in designing smart hydrogels with the structures and properties similar to the interior of extracellular matrix have aroused the interest of researchers as promising cell support scaffolds in the field of tissue engineering. In this study, a novel hydrogel system is developed from natural polysaccharides that are sodium alginate, gelatin and carboxymethyl chitosan through Schiff base cross-linking mechanism. By mixing these three components, the hydrogel system with tunable strength and gelation time was obtained and abbreviated as oxACG hydrogel. Moreover, the obtained hydrogels are biocompatible and can sustain the growth and proliferation of Hela cells after 24 h incubation. The experimental results show that the hydrogel possesses a relatively short gelation time that is around 3 min, high gel strength and negligible cell cytotoxicity. The oxACG hydrogel composite exhibited desirable properties, and they can be used as high-performance multifunctional injectable hydrogels in cell therapy.

Keywords:injectable hydrogels;cell support;biomedical applications;cell friendly environment;natural polysaccharides

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[2] Jose G, Shalumon KT, Chen JP, Curr. Med. Chem., 27, 2734 (2020)

[3] Torgersen J, Qin XH, Li Z, Ovsianikov A, Liska R, Stampfl J, Adv. Funct. Mater., 23, 4542 (2013)

[4] Le Thi P, Son JY, Lee Y, Ryu SB, Park KM, Park KD, Macromol. Res., 28(4), 400 (2020)

[5] Park KM, Joung YK, Park KD, Lee SY, Lee MC, Macromol. Res., 16, 517 (2008)

[6] Fedorovich NE, Alblas J, de Wijn JR, Hennink WE, Verbout AJ, Dhert WJ, Tissue Eng., 13, 1905 (2007)

[7] Tabata Y, Tissue Eng., 9, 5 (2003)

[8] Vermonden T, Censi R, Hennink WE, Chem. Rev., 112, 2853 (2012)

[9] Li Y, Rodrigues J, Tomas H, Chem. Soc. Rev., 41, 2193 (2012)

[10] Kharkar PM, Kiick KL, Kloxin AM, Chem. Soc. Rev., 42, 7335 (2013)

[11] Sivashanmugam A, Kumar RA, Priya MV, Nair SV, Jayakumar R, Eur. Polym. J., 72, 543 (2015)

[12] Wichterle O, Lim D, Nature, 185, 117 (1960)

[13] Tollar M, Štol M, Kliment K, J. Biomed. Mater. Res., 3, 305 (1969)

[14] Kim D, Thangavelu M, Baek JS, Kim HS, Choi MJ, Cho HH, Song JE, Khang G, Macromol. Res., 28, 196 (2020)

[15] Li L, Scheiger JM, Levkin PA, Adv. Mater., 31, 1807333 (2019)

[16] Qu J, Zhao X, Ma PX, Guo B, Acta Biomater., 58, 168 (2017)

[17] Kuchaiyaphum P, Chotichayapong C, Butwong N, Bua-ngern W, Macromol. Res., 28, 844 (2020)

[18] Gomez CG, Rinaudo M, Villar MA, Carbohydr. Polym., 67, 296 (2007)

[19] Wu T, Li Y, Lee DS, Macromol. Res., 25, 480 (2017)

[20] Lou J, Stowers R, Nam S, Xia Y, Chaudhuri O, Biomaterials, 154, 2013 (2018)

[21] Vega SL, Kwon MY, Song KH, Wang C, Mauck RL, Han L, Burdick JA, Nat. Commun., 9, 1 (2018)

[22] Song CK, Kim MK, Lee J, Davaa E, Baskaran R, Yang SG, Macromol. Res., 27, 119 (2019)

[23] Drury JL, Mooney DJ, Biomaterials, 24, 4337 (2003)

[24] Chen QZ, Harding SE, Ali NN, Lyon AR, Boccaccini AR, Mater. Sci. Eng. R-Rep., 59, 1 (2008)

[25] Kim EH, Lim S, Kim TE, Jeon UO, Choi YS, Biotechnol. Bioprocess Eng., 23, 500 (2018)

[26] Baek JS, Carlomagno C, Muthukumar T, Kim D, Park JH, Song JE, Migliaresi C, Motta A, Reis RL, Khang G, Macromol. Res., 27, 558 (2019)

[27] Qu J, Zhao X, Liang Y, Zhang T, Ma PX, Guo B, Biomaterials, 183, 185 (2018)

[28] Feng Q, Xu J, Zhang K, Yao H, Zheng N, Zheng L, Wang J, Wei K, Xiao X, Qin L, ACS Central Sci., 5, 440 (2019)

[29] Tan L, Liu Y, Ha W, Ding LS, Peng SL, Zhang S, Li BJ, Soft Matter, 8, 5746 (2012)

[30] Balakrishnan B, Joshi N, Jayakrishnan A, Banerjee R, Acta Biomater., 10, 3650 (2014)

[31] Ullah F, Othman MBH, Javed F, Ahmad Z, Akil HM, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 57, 414 (2015)

[32] Jeon O, Alt DS, Ahmed SM, Alsberg E, Biomaterials, 33, 3503 (2012)

[33] Prabaharan M, J. Biomater. Appl., 23, 5 (2008)

[34] Yang C, Xu L, Zhou Y, Zhang X, Huang X, Wang M, Han Y, Zhai M, Wei S, Li J, Carbohydr. Polym., 82, 1297 (2010)

[35] Yan S, Wang T, Feng L, Zhu J, Zhang K, Chen X, Cui L, Yin J, Biomacromolecules, 15, 4495 (2014)

[36] Hasan MM, Khan MN, Haque P, Rahman MM, Int. J. Biol. Macromol., 117, 1110 (2018)