The Functional layers with excellent heat-resistence nanofiltration (HRN) property were designed by fixing thermal stabilized quinoxaline diamines (QHDA) on poly (m-phenyleneisophthalamide) (PMIA) substrate through interfacial polymerization. QHDA was used as aqueous monomer while cinnamoyl chloride (CNC), isophthaloyl chloride (IPC) and trimesoyl chloride (TMC) were served as organic monomers to realize the immobilization of thermally stable N heterocycles. The experimental results stated clearly that the compact package reaction of QHDA with TMC lead a raise of thermal stability and salts rejection at high temperature stream. With the optimal TMC and 0.5 wt.% QHDA, the as-developed membrane achieved the excellent NF performance and thermal stability. The rejection of MgSO4 reached to 94.6 % at 30 ℃ and hardly decrease at 90 ℃. The reduction in rejection was satisfactory at high temperatures. The functional layer still showed highly stability after 10 h long-term operation at 90 ℃ with only 2.8 % reduction in MgSO4 rejection. Besides, the excellent Mg2+ and poor Li+ rejection made this membrane have huge potential in the application of extracting Li+ from brine. The separation factor of Mg2+-Li+ at high temperature was satisfactory. Over all, this study offered a prospect technology to exploit heat-resistant membranes for extraction lithium at high temperature.