Atomic force microscopy has been used to image wheat seed starch granules (cultivar Timmo) both in air and under water. In the aqueous environment, the enzyme alpha-amylase was introduced into the solution cell of the microscope and its progressive attack on a starch granule was followed by collecting successive images of the granule. Each image was recorded over a time period of 105 s. The enzyme created a "pin-hole" in the top of the granule, consistent with previous off-line electron microscopy studies.