Synthesis of Alanine and Leucine by Reductive Amination of 2-Oxoic Acid with Combination of Hydrogenase and Dehydrogenase

Hasumi F; Fukuoka K; Adachi S; Miyamoto Y; Okura I

Applied Biochemistry and Biotechnology, Vol.56, No.3, 341-344, 1996

Hasumi F, Fukuoka K, Adachi S, Miyamoto Y, Okura I

Alanine synthesis by reductive amination of pyruvate was performed by the combination of NADH regeneration system and alanine dehydrogenase (AlaDH). The conversion of pyruvate to alanine was 99% after 1 h. Leucine synthesis was also carried out by the combination of NADH regeneration system and leucine dehydrogenase (LeuDH). The conversion of 4-methyl-2-oxovalerate to leucine was 60% after 1.5 h.

Keywords:REGENERATION