Filter paper, carboxymethylcellulase, beta-glucosidase, and xylanase activities were determined and compared to cellulases originating from Aureobasidium sp. (Ab), Penicillium sp. (Pe), and Aspergillus terreus (At). The formation of total reducing sugar was measured as a function of time for the hydrolysis of the native sugar cane bagasse pith using culture filtrates with the same quantity of extracellular protein content from Ab, Pe, At, or from the following mixtures Ab:Pe/Ab:At and Pe:At at different Volumetric ratios. The saccharification progress indicated a synergistic effect for the mixture of all the enzymes, the highest being in the 40:60 relation of Pe and At, respectively. The synergistic action has been assigned to a better balance of endo- and exoglucanases in this system and essentially to the addition of beta-glucosidase and xylanases from At to Pe cellulases.