The effects of reversed micelles on the bis(2,4,6-trichlorophenyl) oxalate (TCPO)-hydrogen peroxide chemiluminescence reaction was evaluated in two micellar systems : (1) hexadecyltrimethylammonium chloride (CTAC) and (2) sodium bis(2-ethylhexyl) sulfosuccinate (AOT), each formed in 1:2 (v/v) 1-butanol/cyclohexane and 1:1 (v/v) chloroform/cyclohexane solvent mixtures with varying amounts of water. Chemiluminescence intensity was increased 6 to 11 times in CTAC and 4.4 to 55 times in AOT reversed micelles. The chemiluminescence efficiency enhancement was caused mainly by the increase of chemical excitation efficiency. The experimental variables that influence the magnitude of the chemiluminescence enhancements were studied. The analytical figures of merit for this chemiluminescence reaction system have been evaluated for the determination of fluorophors. Using a flow injection system, the detection limits for 8-anilino-1-naphthalenesulfonic acid (ANS) were improved 20 and 7 times in CTAC and AOT reversed micellar medium, respectively. The detection limits for Rhodamine B (Rh B) were improved 5 and 50 times in the same reversed micellar medium. The detection linear range and precision were also improved.