This paper presents a novel way to produce poly(diacetylene) thin films of uniform fluorescence on a solid support. The method involves film formation at the air-water interface from a modified diacetylene monomer using innovative spreading, crystallization, and transfer techniques. Pressure-area isotherms for the monomer N-(2,3-dihydroxypropane)pentacosa-10,12-diynoicamide reveal the optimum lateral pressure (10 mN/m) and temperature (40 degrees C) for film production. To prepare films, we apply a 2 mM solution of the monomer in chloroform to the air-water interface, either dropwise or as a nebulized spray. Irradiation with UV light polymerizes the film to its red, fluorescent form. Pressurized nitrogen directed at the subphase surface creates turbulence in the interfacial layer, causing break-up and randomization of crystal domains. After stopping the surface disruption, we transfer the film to silanized glass slides held at 40 degrees C. Heating the transfer slides eliminates holes in the film attributed to condensation of water vapor onto the slides during transfer. The resulting film consists of uniformly distributed crystals of less than or equal to 10 mu m, and it is free of the large cracks and defects commonly observed in solid-supported poly(diacetylene) films. The transferred film has very uniform fluorescence with a coefficient of variance of only 5% in fluorescence intensity based on measurements 1 mm in diameter covering the entire slide (2.5 cm x 7.5 cm).