The conjugation of the [Abu(105,109)] VP3(101-121) peptide sequence from the VP3 capsid protein of hepatitis A virus to synthetic branched polypeptide carriers is described. The establishment of either a disulfide or an amide bond between the peptide and the carrier molecules is reported. The interaction of VP3 conjugates with dipalmitoylphosphatidylcholine (DPPC) mono-and bilayers was studied, and the influence of the different hydrophilicities and peptide orientations on the conjugates is discussed. Results showed an increased interaction with biomembrane models due to the conjugation of VP3 peptide to the described macromolecular structures.