Mono- and di-deprotonated 1,4-dihydroxy-anthraquinones are separately prepared with titration by sodium-ethylate and their properties of persistent spectral hole-burning in ethanol/methanol organic glass at cryogenic temperatures are investigated. Formations of zero-phonon holes are both detected though the burning yield is one (mono-) or two (di-) order(s) of magnitude smaller if compared with undeprotonated case. Spectral profiles of product and educt states indicated non-photochemical nature of the burning process for both. The role of intramolecular hydrogen bondings in hole burning and electronic states in anthraquinone derivatives is discussed.