TOPOGRAPHICAL maps of neuronal connectivity occur in various brain regions(1). In the visual system of birds, retinal ganglion-cell axons from the anterior retina connect to a posterior part of the optic tectum, and posterior retinal axons connect to the anterior part, thereby establishing a point-to-point projection map(2,3). The chemoaffinity theory(4) predicts that the orderly retinotectal projection is generated by a topographical arrangement of molecules. We report here that me have found several genes topographically expressed along the nasotemporal (anterior-posterior) axis in the embryonic chicken retina. Among these, two transcriptional regulators, belonging to the winged-helix family(5) are expressed in a mutually exclusive manner in either the nasal or temporal part of the retina, Misexpression of each factor causes misprojection on the tectum along the rostrocaudal axis, showing that topographical expression of these transcription factors controls formation of the retinotectal map.