The goal of this work was to select higher fungi for the production of enzymes able to produce plant protoplasts. The hydrolytic capacity of various species of wood-destroying fungi was examined to select those with the maximal hydrolytic capacity. The selection criteria included attack on cellulose, and oligo- and polysaccharides by each fungus species. Lenzites elegans, Ganoderma applanatum and Pycnoporus sanguineus produced the richest enzymic mixtures, which were evaluated for their protoplasting ability. L. elegans has the best enzymic mixture able to produce plant protoplasts with the shortest time for maceration of plant tissues, followed by G. applanatum and P. sanguineus. The potential of these fungi and the simplicity of enzyme production suggest the possibility of commercial applications.