Crude proteases produced by Bacillus amyloliquefaciens in solid substrate fermentation (SSF) were utilised for the production of a protein hydrolysate using chick pea as the protein source. These enzymes were found to be better hydrolytic agents than fungal enzymes. The hydrolysate generated was separated on an immobilised metal ion affinity chromatography (Cu2+) column using a volatile elution buffer. Two fractions were rich in the amino acid methionine and could be used as a peptide fortifier in different food products.