Studies on proteolysis of hemoglobin by Alcalase 0.6L are described. Trials were conducted in a batch reactor at different operating conditions. Degrees of hydrolysis in the proteolysates were determined and kinetics of the reaction was considered in detail in relation to substrate concentration, enzyme concentration, temperature, degree of hydrolysis and reaction time. From the results a general kinetic equation for the enzymic hydrolysis of proteins is suggested. The proposed kinetic model allows an easier determination of kinetic parameters using only a few experiments.