화학공학소재연구정보센터
Applied Microbiology and Biotechnology, Vol.52, No.4, 546-552, 1999
Physiology and kinetics of trimethylamine conversion by two methylotrophic strains in continuous cultivation systems
The change of dilution rate (D) on both Methylophilus methylotrophus NCIMB11348 and Methylobacterium sp. RXM CCMI908 growing in trimethylamine (TMA) chemostat cultures was studied in order to assess their ability to remove odours in fish processing plants. M. methylotrophus NCIMB11348 was grown at dilution rates of 0.012-0.084 h(-1) and the biomass level slightly increased up to values of D around 0.07 h(-1). The maximum cell production rate was obtained at 0.07 h(-1) corresponding to a maximum conversion of carbon into cell mass (35%). The highest rate of TMA consumption was 3.04 mM h(-1) occurring at D=0.076 h(-1). Methylobacterium sp. RXM CCMI908 was grown under similar conditions. The biomass increased in a more steep manner up to values of D around 0.06 h(-1) The maximum cell production rate (0.058 g l(-1)h(-1)) was obtained in the region close to 0.06 h(-1) where a maximum conversion of the carbon into cell mass (40%) was observed. The maximum TMA consumption was 2.33 mM h(-1) at D = 0.075 h(-1) The flux of carbon from TMA towards cell synthesis and carbon dioxide in both strains indicates that the cell is not excreting products but directing most of the carbon source to growth. Carbon recovery levels of approximately 100% show that the cultures are carbon-limited. Values for theoretical maximum yields and maintenance coefficients are presented along with a kinetic assessment based on the determination of the substrate saturation constant and maximum growth rate for each organism.