화학공학소재연구정보센터
Applied Microbiology and Biotechnology, Vol.53, No.5, 601-604, 2000
Biotransformation of methyl ent-17-hydroxy-16 beta-kauran-19-oate by Rhizopus stolonifer
Methyl ent-17-hydroxy-16 beta-kauran-19-oate was fed to a 2-day-old culture of the fungus Rhizopus stolonifer, fermenting at room temperature (25 degrees C) in an orbital shaker (21). After 11 days, both broth and mycelia were extracted with ethyl acetate. Two novel compounds were isolated from this experiment: methyl ent-9 alpha; 17-dihydroxy-16 beta-kauran-19-oate and methyl ent-7 alpha, 17-dihydroxy-16 beta-kauran-19-oate. Their structures were fully confirmed by spectroscopic methods.