화학공학소재연구정보센터
Science, Vol.273, No.5274, 503-507, 1996
Lymphocyte Apoptosis - Mediation by Increased Type-3 Inositol 1,4,5-Trisphosphate Receptor
B and T lymphocytes undergoing apoptosis in response to anti-immunoglobulin M antibodies and dexamethasone, respectively, were found to have increased amounts of messenger RNA for the inositol 1,4,5-trisphosphate receptor (IP(3)R) and increased amounts of IP(3)R protein. Immunohistochemical analysis revealed that the augmented receptor population was localized to the plasma membrane. Type 3 IP(3)R (IP(3)R3) was selectively increased during apoptosis, with no enhancement of type 1 IP(3)R (IP(3)R1). Expression of IP(3)R3 antisense constructs in S49 T cells blocked dexamethasone-induced apoptosis, whereas IP(3)R3 sense, IP(3)R1 sense, or IP(3)R1 antisense control constructs did not block cell death. Thus, the increases in IP(3)R3 may be causally related to apoptosis.