화학공학소재연구정보센터
Science, Vol.277, No.5323, 221-225, 1997
Inducible Expression and Phosphorylation of Coactivator Bob.1/Obf.1 in T-Cells
BOB.1/OBF.1 is a transcriptional coactivator that is constitutively expressed in B cells and interacts with the Oct1 and Oct2 transcription factors. Upon activation of Jurkat T cells and primary murine thymocytes with phorbol esters and ionomycin, BOB.1/OBF.1 expression and transactivation function were induced. BOB.1/OBF.1 was phosphorylated at Ser(184) both in vivo arid in vitro, and this modification was required for inducible activation. Mutation of Ser(184) also diminished transactivation function in B cells, suggesting that the activating phosphorylation that is inducible in T cells is constitutively present in B cells. Thus, BOB.1/OBF.1 is a transcriptional coactivator that is critically regulated by posttranslational modifications to mediate cell type-specific gene expression.