A synthetic mixture of fructose, mannitol and sorbitol was continuously separated by a chromatographic method using a cation-exchange resin (Dowex 50W-X8) in its Ca2+-form as the stationary phase. An annular chromatograph (AC) was used to achieve a continuous mode of operation. Distribution and mass transfer coefficients of the three substances as well as bed properties were obtained by batch chromatography. The separation was simulated mathematically in terms of an approximate linear chromatographic theory was applied to the modeling of the behavior of the continuous separations. The influence of rotation rate, column loading, eluent flow rate and feed concentration on the resolution of the individual peaks were investigated.