The extraction of lincomycin was carried out with different types of neutral donor extractants, and the stripping of extracted lincomycin was performed with hydrochloric acid. The effects of such factors as concentrations of solvent and lincomycin, pH of solution, temperature, and additives on the extraction of lincomycin was investigated. The relationships between the electron-donating ability of extractant and extraction selectivity plus the ease of stripping with hydrochloric acid are discussed. A new solvent system is suggested for the extraction of lincomycin from aqueous solutions.