The influence of the molecular geometry is of fundamental importance for a better understanding of the photoinduced electron transfer mechanism. Because of their typical molecular structures, cyclophane rings have proved to be suitable for this purpose as electron acceptor molecules adsorbed under an amphiphile monolayer. We used a pyrene-labelled phospholipid derivative both as molecular anchor for the cyclophane ring and electron donor molecule. The co-spreading technique was used to prepare the complex monolayers. Surface pressure and surface potential measurements have indicated similar monolayer behaviour as with dimyristoylphosphatidic acid as anchor molecule, leading to the same parallel orientation of the molecular plane of the cyclophane ring with respect to the water surface. Surface reflection has revealed a change in orientation of the pyrene moiety during compression, but no relevant dimer formation was observed. Fluorescence spectra were almost of excimer emission nature only. Steady state fluorescence quenching of the pyrene depends on the pyrene/cyclophane co-spreading ratio.