Biotechnology and Bioengineering, Vol.44, No.1, 21-28, 1994
Cultivation of an L-Lactate Dehydrogenase Mutant of Bacillus-Stearothermophilus in Continuous-Culture with Cell Recycle
Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD-15, on sucrose at 70 degrees C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system.