Polyglutamates with mesogenic and photochromic azobenzene moieties in the side chain have been investigated at the air-water interface by means of UV-Vis spectroscopy. The spectra recorded upon compression of the monolayer indicate a domain-like structure. The chromophores in the side chains show a spontaneous aggregation, which stabilizes the domains, Application of a surface pressure gives rise to a deformation of the polymers in such a way that the chromophores in the side chains are directed towards the gas phase, The extent of the deformation strongly depends on the architecture of the polymer, especially on the sparer used to tether the chromophore to the polymer backbone. The deformation is retained in the transfer process and determines the structure and the properties of the LB film.