The 3-morpholinylphthalimide and phthalanhydride derivative films deposited on hydrophobic substrates or substrates covered with Langmuir-Blodgett (LB) films have been investigated by linear dichroism measurements, X-ray diffraction, and electron microscopy methods. The structure and quality of the films depend on the deposited compound structure, the deposition rate and on the substrate temperature. Amorphous and polycrystalline films of the substances investigated were found to be formed both on the hydrophobic and LB film covered substrates at a room temperature. When the substrate temperature was raised to 323 K 4-(4-morpholinyl)-7-phenyl-1,3-isobenzofurandione only tends to grow with preferably orientation on the LB film of the mixture of 7-[(4-acetyloxy)phenyl]-4-(4-morpholinyl)-2-octadecyl-1H-isoindole-1,3-(2H)-dione and the stearic acid covered substrate.