Two-dimensional organic and organic/inorganic networks containing functional groups or nanoparticles were formed using Langmuir monolayer technique. Kinetics of chemical transformations in monolayers of epoxy oligomer on the surface of aqueous solutions of oligoamine or 12-phosphotungstic acid were studied. Using combination of film balance, FTIR spectroscopy and sol-gel techniques, we found that molecules of oligoamine and heteropolyacid are immobilized in network of epoxy polymer. Nanoparticles of polyacid introduced in the epoxy matrix can not be withdraw from the monolayer neither by neutralization of subphase leading to decomposition of polyanions, nor by extraction of polyanions with strong solvents. The compositions of 2D networks of epoxy polymers with each reactive agent were evaluated. Such systems with enhanced stability can serve as a matrix for immobilization of molecules and nanoparticles with the desired chemical, physical, and biological behavior.