Investigations have been carried out to probe the conformations of tethered poly(ethyleneglycol) (PEG) chains anchored on polystyrene (PS) latex particles in presence of an anionic surfactant, sodium dodecyl sulfate (SDS) and two inorganic salts, sodium and potassium chloride. For this purpose, PS latex particles were labeled with pyrene and mononaphthayl PEG ester. Distance dependent nonradiative energy transfer from naphthalene moieties to pyrene moieties was used as a ruler in this study. Results showed that the separation distance between the acceptor and the donor changes with external stimuli. Analysis of the results suggested that there is considerable contraction of the polymer chain upon interaction with salts and surfactant below its critical micelle concentration. The study also reveals that SDS interacts with PEG chains bellow its critical micellar concentration, probably due to binding of SDS counterions to PEG chains.