Polymers capable of responding to both temperature and pH changes were synthesised by radical copolymerisation of N-isopropyl-acrylamide (NIPAAm) and N-methacryloyl-L-leucine (MALEU) in different NIPAAm/MALEU molar ratios (15.1, 10:1, 2:1 and 1:1). The polymers were characterised by Size Exclusion Chromatography, acid-base titrations, FT-IR and H-1-NMR analyses. Their solution behaviour was studied in 0.1 M NaCl and in citrate buffers (pH 3.0, 4.5, 5.0 and 6.0). At pH 4.0 and 4.5 the cloud point temperature (T-CP) of copolymers was depressed proportionally to their MALEU content. In contrast, at pH 5.0 and 6.0 the phase transition temperature increased linearly. The behaviour of the copolymers with NIPAAm/MALEU ratios 15:1 and 10:1 was investigated also between pH 3 and 11 in 0.1 M NaCl. In both cases a sharp increase in T-CP was observed around pH 4-5. The T-CP vs. pH curve was linearised and the acidity constants were determined by the linearisation procedure. Furthermore, it was possible to demonstrate that the phase transition temperature increases linearly with the ionic content of the polymers.