Biotechnology Letters, Vol.17, No.1, 13-18, 1995
Cloning of Escherichia-Coli Penicillin-G Acylase Gene with Mini-Mu Containing a Plasmid Replicon
An in vivo cloning system based on mini-Mu derivatives was used for cloning of E. coli penicillin G acylase gene (pac). We have constructed several recombinant clones producing penicillin G acylase and some of them exhibit approximately two times higher activity than original strains.