Polymer, Vol.42, No.5, 2263-2266, 2001
Influence of cross-linking monomer and hydrophobic styrene comonomer on stereoselective esterase activities of polymer catalyst imprinted with a transition-state analogue for hydrolysis of amino acid esters
Polymer catalysts, cross-linked with N,N'-ethylene (C-2) {or butylene (C-4)}-bisacrylamide containing L-histidine and quaternary trimethylammonium groups were imprinted with a racemic transition-state analogue of phenyl 1-benzyloxycarbonyl-3-methylpentylphosphonate for the hydrolysis of p-ni(rophenyl N-(benzyloxycarbonyl)-L (or D)-leucinate {Z-L (Dr D)-Leu-PNP}. Maximal stereoselectivity (L/D = 8.4) was obtained by using N,N'-C-4-bisacrylamide cross-linked polymer catalyst, which was copolymerized with hydrophobic styrene monomer.