Biotechnology Letters, Vol.17, No.2, 171-176, 1995
Effect of Chemical Modification of Isoenzyme-A and Isoenzyme-B from C-Rugosa on Their Activity and Stability
Lipases A and B from C.rugosa have been chemically modified with octanoyl chloride or polyethylene glycol (molecular weight : 750 or 5000). Although the hydrolytic activity was diminished, modification gave rise in some cases to preparations with increased synthetic activity in hexane. Furthermore, PEG(5000)-lipases A and B were significantly more stable (up to 20-fold) at alkaline pH values than the native enzymes.