Coleonol was produced in callus culture; the kind and level of phytohormones, glycine, casein hydrolysate and sucrose content of the medium differently influenced growth and product formation. Maximum specific growth rate was obtained in medium containing 7% sucrose. Biomass production was highest with 4 ppm of NAA. Maximum product (0.075% of dry cells) was formed in medium containing 0.5 ppm IAA and IBA each, 5 ppm glycine, 200 ppm casein hydrolysate and 7% sucrose.