A recombinant strain of Saccharomyces cerevisiae secreting bacterial cellodextrinase was constructed. The Ruminococcus flavefaciens cellodextrinase gene (celA) was inserted between a yeast expression-secretion cassette and yeast gene terminator, and cloned into a yeast-centromeric shuttle vector. Enzyme assays revealed growth-associated production of biologically active cellodextrinase by S. cerevisiae transformants.