High production levels of recombinant cholera toxin B subunit in Escherichia coil were obtained with the design of an efficient fed-batch process and control strategy. The fed-batch results demonstrated a biomass production of 58 g/L (Cell Dry Weight) attaining production levels of heterologous protein of 4.7 g/L in the intracellular fraction, 0.96 g/L exported into the periplasm and 0.27 g/L secreted into the culture supernatant.