The fate of Vibrio cholerae was studied during anaerobic digestion of human night soil (HNS). In an experimental digester (VFA congruent to 8000 mg/l and pH congruent to 6.4), counts of V. cholerae declined rapidly and the counts were below detectable limits within 20 d. In the control digester (VFA congruent to 500 mg/l and pH 7.6), a four log reduction in count was achieved, however, the pathogen was not completely eliminated. T-90 values for the experimental and control digesters were 1.53 and 2.63 d, respectively.