Development of a double exponential model for determining the intrinsic kinetic parameters for factor X activation by tissue factor-factor VIIa (TF:VIIa) complex, during the complete course of the reaction in a flow reactor is described. The model data reveal that the factor X activation rate constant K-1 gradually increased from 0.0225 to 0.0456 min(-1) as the shear rate increased from 50 to 3000 sec(-1), whereas the factor Xa inhibition rate constant K-2 increased dramatically from 0.307 to 1.09 min(-1) for a similar increase in shear rate.