Biotechnology Letters, Vol.22, No.14, 1165-1167, 2000
Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E-coli
To study the characteristics of recombinant thin aggregative fimbriae of salmonella and to develop a vaccine for salmonella infections, the AgfA subunit gene was amplified from Salmonella entiritidis using PCR. Maltose binding protein (MBP)-AgfA fusion protein was over-produced in E. coli and purified. Antibody against MBP-AgfA was prepared and its immunogenicity was studied.