Biotechnology Letters, Vol.22, No.24, 1963-1966, 2000
A two-site immunometric assay for the detection of the expression level of alpha(2)(C2)-adrenergic receptor produced in Escherichia coli
Human alpha (2)(C2)-adrenergic receptor was expressed in Escherichia coli as a fusion protein with Bacillus circulans var. alcalophilus cyclomaltodextrin glucanotransferase. For the determination of the expression level (0.6 mg of solubilized fusion protein l(-1) of E. coli culture), a two-site immunometric assay based on two monoclonal antibodies with different epitopes was developed.